Coding
ccdB

Part:BBa_K145151:Design

Designed by: Jonas Demeulemeester   Group: iGEM08_KULeuven   (2008-08-07)

ccdB coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


Design Notes

This part was made with a PCR of template BBa_P1010. The primers used were: 

primer 1: CATCATGAAT TCGCGGCCGC TTCTAGATGC AGTTTAAGGT TTACACC
primer 2: CTGCAGCGGC CGCTACTAGT ATTATATTCC CCAGAACATC AGG

Source

P1010

References